Conjugated to fluorescent tags
WebIf your protein is epitope tagged (e.g., 6xHis, HA), you may be able to take advantage of a fluorophore conjugated primary antibody specific to the epitope tag. A primary antibody directly conjugated to a fluorophore does not require the use of a fluorophore-conjugated secondary antibody. WebOne solution that enables easy detection is to genetically fuse protein and peptide tags to the protein of interest. Tagged proteins can be used for purposes such as immunoprecipitation, microscopy, protein purification, Western blotting, protein arrays, etc. Proteins fused to GFP are called GFP-tagged proteins or GFP-fusion proteins.
Conjugated to fluorescent tags
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WebAbstract. Nanoparticles primarily consisting of π-conjugated polymers have emerged as extraordinarily bright fluorescent tags with potential applications in biological imaging and sensing. As fluorescent tags, conjugated polymer nanoparticles possess a number of advantageous properties, such as small particle size, extraordinary fluorescence ... WebJan 1, 2010 · Anti-His tag antibodies are available directly conjugated to HRP and AP - making the use of secondary antibodies in Western blotting redundant For all your multicolor flow needs anti-His tag antibodies are available conjugated to a large selection of Alexa Fluor®, DyLight® and conventional dyes
WebAug 2, 2015 · - an immunofluorescent assay technique in which antibody that is conjugated with a fluorescent tag is added directly to unknown antigen that is fixed to a microscope slide - after incubation and a wash step, the slide is read using a fluorescence microscope - best suited to antigen detection in tissue or body fluids Indirect Immunofluorescent Assay WebThe conjugation of dyes to cysteine residues provides site-directed labeling with defined degree of labeling (DOL). Conjugated Nanobodies are suitable for quantitation assays. …
WebHis-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Our His-tag antibodies also come with the option of being HRP, biotin, or fluorophore conjugated to meet all your research needs. WebThey can be fused to a protein in transgenic cells or animals, conjugated to an antibody, or even used as a substrate in enzymatic reactions. Before selecting a fluorescent protein for any of these applications, there are a number of key considerations to keep in mind.
WebActivated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding …
WebFluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell … supino no smithWebNi-NTA-Atto conjugates provide a valuable alternative to traditional antibody immunodetection for specific and sensitive detection of His-tagged fusion proteins. Use … barbecuing meaning in hindisupino raeWebPhalloidin belongs to a class of toxins called phallotoxins, which are found in the death cap mushroom (Amanita phalloides).It is a rigid bicyclic heptapeptide that is lethal after a few days when injected into the bloodstream. The major symptom of phalloidin poisoning is acute hunger due to the destruction of liver cells. It functions by binding and stabilizing … supino roma 3WebFluorescent tags are fluorophores conjugated to antibodies or proteins. Each fluorophore is excited at a particular wavelength and combining fluorophores with unique emission … barbecuing asparagusWebDec 10, 2024 · Importantly, the FSEC profiles were superimposable between the two fluorescent tags ... To this end, purified sybody 44 (Sb44) was conjugated to a solid … supi noriWebWe offer quality antibodies specific to FLAG tag that can be used in a variety of research needs. Fluorescent and enzyme-conjugated versions of our FLAG Tag antibodies are available for your added convenience. All antibodies listed here recognize a similar epitope to the ANTI-FLAG® M2 antibody of Sigma-Aldrich. supino roma