NettetSterilizing your loop or needle 1. Hold the inoculating loop in your dominant hand like a pencil. See figures 2 and 3. To sterilize, place it in the incinerator for AT LEAST 10 SECONDS (or flame it at an angle so that the wire is along the margin of the inner blue flame. The ENTIRE WIRE must be heated RED HOT). Watch the clock for the time. Nettet12. apr. 2024 · If a hot incinerator is available, the loop may be sterilized by holding it inside the incinerator for 5 to 7 seconds. Once sterile, the loop is allowed to cool by holding it still. Do not wave it around to cool it or blow on it. When manipulating bacteria, transfer loops are usually held like a pencil.

What does inoculation loop mean? - Definitions.net

Nettetfor 1 dag siden · A subculture was established by inoculating the seed culture into fresh 5 ml of fresh LB broth (2% volume) in a 50-ml centrifuge tube and grown for 2 hours with shaking at 220 r.p.m. to reach ... graphics driver update win 10

Lab 2 (Ch. 1) Flashcards Quizlet

NettetSterilizing the Inoculating Loop or Needle. Hold the inoculating loop in your dominant hand like a pencil. To sterilize, place it in the Bunsen burner for at least 10 seconds. The entire wire must be heated red hot. Use the center blue region of the flame (not the top or bottom of the flame). Watch the clock for the time. Students tend to count ... Nettet1. Cool your inoculating loop completely before you use it. Hot loops will kill microbes and potentially can create aerosols when the hot loop touches the agar. 2. Hold the inoculating loop like a pencil instead of a hammer. The motion of moving the inoculating loop comes from the action of your fingers and wrist, rather than from the elbow. 3. Nettetfor 1 dag siden · Exponential phase cultures were used to inoculate the cell culture tubes with the glass slides at an OD 600 = 0.01 (for example, 20 µL of a culture at OD 600 = 0.5 was used to inoculate the tube ... graphics driver version nvidia