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Pclbw cox8 egfp mcherry

Splet23. avg. 2024 · 131 COX8-EGFP, and obtained the COX8-EGFP expression cells. After screening by G418 132 for 2 weeks, the stably transfected cell line was obtained. The COX8-EGFP-mCherry-133 ACTIN co-expressing HeLa cell line was further established based on the COX8-EGFP 134 expressing cells with lentiviral transfection system. From the as … SpletpCLBW cox8 EGFP mCherry was a gift from David Chan (Addgene plasmid # 78520 ; http://n2t.net/addgene:78520 ; RRID:Addgene_78520) For your References section: …

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Splet15. mar. 2024 · pCLBW cox8-EGFP-mCherry plasmid were transiently transfected into MC38 PINK1 overexpressing cells and their parental controls. After 24 h, cells were … SpletArticle Snippet: The pCLBW retroviral vector was used to ectopically express Cox8-EGFP-mCherry (a gift from D. Chan (California Institute of Technology); Addgene plasmid … dj and paulina https://road2running.com

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SpletGeneration of stable COX8-EGFP-mCherry reporter SH-SY5Y cells The COX8-EGFP-mCherry sequence was obtained from the pCLBW COX8-EGFP-mCherry construct [39] (a gift from Prof. David Chan (Caltech, Pasadena, CA), Addgene plasmid #78520) through restriction enzyme digestion with ApaI and EcoRI, and was ligated into the SpletThe pCLBW cox8 EGFP mCherry promotor is combined with a vector sequence and a gene in the plasmid vector map. Conjugate The Green Fluorescent Protein (eGFP) of pCLBW … Splet01. mar. 2024 · Cox8-EGFP-mCherry Fluorescence microscopy Mitochondria Lysosome 1. Introduction Autophagy (or macroautophagy) is a catabolic process that degrades … ترجمه به زبان عربی

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Category:miR-379 deletion ameliorates features of diabetic kidney disease …

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Pclbw cox8 egfp mcherry

miR-379 deletion ameliorates features of diabetic kidney disease …

Splet24. jul. 2024 · pCLBW cox8 EGFP mCherry. catalog : 78520. more info or order : Addgene product webpage. citations: 15. Reference; Li C, Li L, Yang M, Yang J, Zhao C, Han Y, et al. PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy. SpletpCLBW cox8 EGFP mCherry Citations (15) Plasmid Article: Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on …

Pclbw cox8 egfp mcherry

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SpletCOX8-EGFP-mCherry-ACTIN co-expressing HeLa cellline was further established based on the COX8-EGFP expressing cells with lentiviral transfection system. From the as-constructed HeLa cells, we could monitor the cell activities in real time using the living cell workstation. As shown inFigure 1C, with the SpletHover: Reveals additional information for enzyme cut sites, ORFs, feature descriptions, and primer details. Select: Click one location on the plasmid and then click on a second …

Splet13. okt. 2024 · EGFP-mCherry-COX8 positive cells were determined using untransfected controls (gate: FITC). Measurements EGFP-mCherry-COX8 were made using dual … Splet04. jan. 2024 · WT and miR-379KO MMC MMC treated with NC siRNA or Fis1 siRNA were transfected with pCLBW-cox8-EGFP-mCherry. and then treated with HG (25 mM) or NG (5.5 mM) for 5 days at 37 °C and 5% CO. 2. Upper ...

Splet06. sep. 2024 · 课题组构建了一个双荧光报告系统（GFP靶向定位于线粒体cox8，mCherry靶向细胞核基因actin），可用于实时原位观测线粒体变化（图1）。. 研究将该系统运用电 … Splet28. jan. 2024 · The principle of using Cox8-EGFP-mCherry to monitor mitophagy. When cells are expressing Cox8-EGFP-mCherry either by transient transfection with a Cox8-EGFPmCherry plasmid (available at Addgene ...

SpletE(0)GFP-mCherry shows ideal properties for FLIM detection of FRET and yields high accuracy both in vitro and in vivo. Furthermore, the recently introduced phasor approach …

SpletThe principle of using Cox8-EGFP-mCherry to monitor mitophagy. When cells are expressing Cox8-EGFP-mCherry either by transient transfection with a Cox8 … djane dubaiSpletCox8 Egfp Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ترجمه به انگلیسی جملهSplet22. avg. 2024 · A pCDNA3.1 encoding for COX8- 157 EGFP-mCherry was generated by subcloning the COX8-EGFP- 158 mCherry coding region from the pCLBW plasmid, a gift from D. Chan 159 (Addgene plasmid #78520), and transfected into U2OS cells using 160 Fugene HD (Promega Madison, WI) [30]. ترجمه به فارسی boredSpletArticle Snippet: The tandem mitochondrial-targeted mCherry-eGFP mitophagy reporter plasmid (pCLBW-cox8-EGFP-mCherry) was a gift from David Chan (Caltech, Pasadena, … djangoSpletProduct Description. pETDuet-1-SopB-EGFP-Ssb-mCherry is available at Gentaur for Next week Delivery. Precision: The pETDuet promotor is combined with a 1 vector sequence and a SopB gene in the plasmid vector map.. Assay procedure summary: Gentaur supplies 2ug of lyophilized ETDuet-1-So Plasmid in powder form at 4°C to be centrifuged at … djane rosehttp://www.biofeng.com/adjy/zhili/pCLBW%20cox8%20EGFP%20mCherry.html djanfouSplet24. nov. 2024 · Pancreatic β-cell dysfunction is caused by, among other things, the destruction of mitochondria by oxidative stress [10,12, 13], as well as the activation of the: hexosamine pathway, nuclear... ترجمه به تركي استانبولي