Phnshp45
WebbThe PCR based mapping of mcr-1 genetic context in our isolates revealed that almost all amplified genes were similar to pHNSHP45, except the truncated tnpA gene, missing IsApl1. Globally, plasmid analysis of various Incompatibility types has shown that IsApl1 is usually absent in IncX4 type plasmid while in Incl2 type plasmid, it is either absent or … WebbPlasmid Transformation Assays. The competent cells including E. coli DH5α containing pUC19-mcr-1 and E. coli C600+pHNSHP45 were prepared and used for transformation assay following the protocol. 14 Mixing 100 μL of chemically competent cells with 10 μL of plasmid pCas9-m1 or pCas9-m2 incubated the reaction mixture on ice for 30 min, and …
Phnshp45
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Webb15 feb. 2024 · pHNSHP45 KP347127 I2 E. coli Pig China [1] pEC019 KY471145 I2 E. coli Chicken Korea [2] pEC111 KY471146 IncX4 E. coli Pig Korea [2] pEC006 KY471144 I2 E. … Webb60 kb in-size, and were similar to pHNSHP45 (Fig. 1), the original sequenced mcr-1 plasmid published in 2015 [5]. The three IncI2 plasmids shared a common plasmid backbone, however, in the case of pPC11 the mcr-1 cas-sette [17] was located in an inverted orientation com-pared to the others (data not shown). Additionally, the
Webb7 jan. 2016 · An analysis of the alignments revealed that sequences of three of these contigs ( E coli strains EC5, EC7, and EC13) share a region of about 14 Kb identical to … Webb23 feb. 2024 · TEXT. The discovery of the plasmid-mediated colistin resistance gene mcr-1 has captured global attention. Unlike colistin resistance raised by chromosomal mutations, the plasmid carrying the mcr-1 resistance gene can be rapidly disseminated through horizontal gene transfer. Since the first report of plasmid pHNSHP45 carrying the mcr-1 …
WebbThe Lancet Infectious Diseases 2016 16, 283-284DOI: (10.1016/S1473-3099(16)00012-8) 21 Blast comparison with pHNSHP45 showed 100% similarity only in a short, 2604 bp region that included mcr-1 (1626 bp) and a truncated ISApI1 mobile element that did not include the transposase encoding Webb1 juni 2024 · Global dissemination of the mobile colistin resistance mcr-1 is of particular concern as colistin is one of the last-resort antibiotics for the treatment of severe …
Webb16 okt. 2024 · Newport strains ASGD2 to pHNSHP45-2 revealed that this plasmid aligned very well with pHNSHP45-2 except that a region containing the tra genes, which were …
Webb8 jan. 2016 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … can distilled water be an effective bufferWebbDOI: 10.1016/S1473-3099(16)00010-4 Corpus ID: 36290610; Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella … fish puppyhttp://www.cpam-cmi.com/article/imagetext-detail/630 can distilled water be used for drinkingWebb28 nov. 2016 · The insertion sequence ISApl1 occurs in both pHNSHP45 and pA31-12 is absent in the plasmid pE15017, which is validated by PCR detection coupled with Sanger … can distilled water give you diarrheaWebb19 dec. 2016 · Compared with the original mcr-1-bearing IncHI2 plasmid pHNSHP45-2, 23 three regions in which antibiotic resistance genes were observable were found to exhibit structural differences (Figure S2). First, the IS Apl1 - mcr-1 - orf region located between terF and the hypothetical CDS was absent in pHYEC7-IncHI2, indicating that mcr-1 could … candis steenbergen rate my profWebb6 feb. 2024 · Except for a 3,684 bp region that was also present in a pHNSHP45-2-like plasmid and included the ISApl1 and mcr-1, the entire nucleotide sequence showed … can distractor throw rocksWebb21 okt. 2024 · The pHNSHP45 possesses a typical IncI2-type backbone encoding replication, horizontal transfer, maintenance, and stability functions. Citation 1 The … can distilled water be used in an aquarium