Protein fitc labeling protocol
Webb5e5 of PBMCs were stained with AF555-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HA2H7) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF555 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested). Protocol WebbThey suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in DMSO) per 1 ml of protein solution. Final molar F/P ratio was 0.06. In another …
Protein fitc labeling protocol
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WebbFITC Mouse IgG1, κ Isotype Ctrl (FC) Verified Reactivity Human Antibody Type Recombinant Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. … Webb产品概述. Protein FITC Labeling Kit ab288089 (previously known as EZLabel Protein FITC Labeling Kit K832) provides an easy way to label proteins with Fluorescein …
WebbPROTEIN LABELING WITHFLUORESCEIN ISOTHIOCYANATE(FITC) Fig: Chemical structure of FITC Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to … WebbCD9-HF2H2, 10 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to …
WebbFITC anti-human CD59 Antibody - CD59 is a 19-25 kD glycosylphosphatidylinositol ... single chain GPI-linked membrane protein, 19-25 kD Distribution All cell types. ... *These products may be covered by one or more Limited Use Label Licenses (see the … Webb14 okt. 2024 · Protein FITC Labeling Protocol Protein Solution Preparation: - The volume of protein solution should not exceed 100 µl. For best results, use 100 µl of ~5-10 mg/ml …
Webb1. LABELING PROTOCOL You may scale this procedure up or down, maintaining the same molar ratios of reagents. It is important to consider that the number and surface …
Webb30 sep. 2024 · For optimal labeling, use 400 pmol of CyDye per 50 μg of protein (see Note 7 ). Mix well by vortexing and centrifuge at 14,000 g for 10 s. Incubate the mixture on ice … ectoine activityectoin hippomedWebb8 okt. 2013 · Hey Cindy, Here is the protocol for preparation and imaging of bacteria for fluorescence microscopy (Growth Curve protocol--see Step 9 under Section II). I also … ectoin® rhinitis nasal sprayWebb2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) and negative control protein … ectoine indicationWebbTake a day zero CSFE sample to determine initial labeling. Run cells immediately or fix in 1-4% paraformaldehyde. Culture: Culture cells in appropriate growth media and take … concrete patios and walkways near meWebbFormat Supplied as: Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized. Formulation: Phosphate buffered saline Reconstitution: Reconstitute with 1 ml distilled water Isotypes: FITC reagent: IgG2a (RAT) RPE reagent: IgG2a (RAT) Preparation Preservative: Expiration: ectokaryothecaWebb3. Dissolve FITC in DMSO to a concentration of 1 μg/μl. The reactive FITC molecule is unstable and should be used immediately after it has been solubilized. Discard any … concrete patios kelowna