WebPSTK specifically phosphorylated the seryl moiety on seryl-tRNA [Ser]Sec and, in addition, had a requirement for ATP and Mg 2+. Proteins with homology to mammalian PSTK occur in Drosophila, Caenorhabditis elegans, Methanopyrus kandleri, and Methanococcus jannaschii, suggesting a conservation of its function across archaea and eukaryotes that synthesize … Web21 Feb 1994 · Crystallization of the Seryl-tRNA Synthetase: TRNA-Ser Complex of Escherichia Coli Price, S., Cusack, S., Borel, F., Berthet-Colominas, C., Leberman, R. (1993) FEBS Lett 324: 167; A New Crystal Form of the Complex between Seryl-tRNA Synthetase and tRNA-Ser from Thermus Thermophilus that Diffracts to 2.8 Angstroms Resolution

The unique tRNA Sec and its role in selenocysteine biosynthesis

WebSeryl-tRNA synthetase (SerRS) was the first class II aaRS whose crystal structure has been solved,[21] and provided the structural basis for the aaRS classification in two distinct classes, based on the conserved signature motifs[22] and … The activity of SA-β-gal in SerRS-overexpressing BJ cells was determined using 5-bromo-4-chloro-3-indolyl P3-D-galactoside (X-gal) following the protocol described in a kit (Beyotime, China). SA-β-gal-positive cells were counted under a microscope and expressed as the % of total cells. See more HeLa 1.2.11, HeLa VST and 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/L glucose (Biological … See more HeLa cells (6×106 cells) stably expressing SerRS or stably transfected with an empty vector were injected subcutaneously into six-week-old female NOD/SCID mice to establish a mouse … See more Nuclear fractionation was carried out as previously described by Xu et al.28Cells were harvested after 24 h, and the cytoplasmic and nuclear fractions were separated and extracted by using a NE-PER Nuclear and … See more For the construction of the pFLAG-SerRS, pFLAG-TRF1 and pcDNA6c-POT1 plasmids, human SerRS, TRF1 and POT1 genes were cloned from HEK293T cells by RT-PCR and inserted … See more planetenkonstellation 2021

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Web21 Feb 1994 · Crystallization of the Seryl-tRNA Synthetase: TRNA(Ser) Complex of Escherichia Coli Price, S., Cusack, S., Borel, F., Berthet-Colominas, C., Leberman, R. (1993) FEBS Lett 324: 167; A New Crystal Form of the Complex between Seryl-tRNA Synthetase and tRNA(Ser) from Thermus Thermophilus that Diffracts to 2.8 Angstroms Resolution WebLegend. Settings. Analysis Web9 Jan 2024 · Seryl-tRNA synthetase is responsible for the attachment of serine to the 3’-ends of tRNA Ser, which takes part in the protein synthesis and formation of respiratory … planett job station lieusaint